The Bio-utilization of Used and Unused car Lubricants by Autochthonous Microorganisms

The determination of the bio-utilization used and unused car lubricants; engine oil, hydraulic transition oil were analyzed using viable counts spectrophotometric analysis. samples collected with 10 sterile containers from different mechanic workshops in Calabar; Unical workshop, designated as UNICAL Workshop (A), Akim (B), Mount Zion (C), Etta Agbor (D), Ekpo Abasi (E). analysis revealed absorbance ranging 2.16 to 2.69nm for 0.57 1.21nm 1.96 2.40nm oil. total heterotrophic ranged 1.2x1010 CFU/ml 4.0x1010 CFU/ml; 1.0x1010 2.2x1010 Transition 2.5x1010 CFU/ml. THB oils had an average count 1.0x10110 enumeration fungal showed lower oil; 1.5x1010 1.0x1010CFU/ml 1.4x1010 very much counts. identification processes presence Pseudomonas spp. (6.5%), Bacillus (13%), Streptobacilli (21.7%), Micrococcus Actinomyces (8.6%), Nocardia (4.3%), Staphylococcus (10.8%), Listeria Serratia (2.1%) Lactobacillus (2.1%). While isolates identified Aspergillus niger (75%), flavus (13.8%), lentulus (5.52%), Candida (2.7%) Curvularia (2.7%). This result suggests that these isolated organisms lubricants can use a source carbon energy, could be explored environmental biodegradation hydrocarbons bioremediation polluted sites by oils. Due medical importance organisms, care should taken while working avoid opportunistic infections.